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anticd133 alexa fluor 488  (R&D Systems)


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    Structured Review

    R&D Systems anticd133 alexa fluor 488
    Anticd133 Alexa Fluor 488, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anticd133+alexa+fluor+488/Human+CD133+Alexa+Fluor%C2%AE+488-conjugated+Antibody/pm41616062-259-72-77
    Average 94 stars, based on 3 article reviews
    anticd133 alexa fluor 488 - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Negative Control:

    Article Title: Monitoring glioblastoma extracellular vesicle evolution using a nanodiagnostic platform to detect glioma stem cells driving recurrent disease.
    Article Snippet: .. Positive particles were gated based on a negative control of PBS or the respective fluorescent antibody to adjust for background fluorescence. sEV immunofluorescent staining for nanoFCM Approximately 1 × 109 total sEV particles were incubated with either anti- ATP1B2 (PA526279, Thermo Fisher Scientific), anti- EAAT2 (NOVNBP120136, Novus), anti- CD9 APC (17- 0098- 42, Thermo Fisher Scientific), anti- CD24 (ab134375, Abcam), anti- EGFR (ab231, Abcam), anti- CD44 Alexa Fluor 488 (103016, BioLegend), or antiCD133 Alexa Fluor 488 (FAB11331G, R&D Systems) in 50 μl of PBS, for 1 hour at 37°C. .. Labeled sEVs were diluted with 1 ml of PBS and ultracentrifuged at 110,000g for 30 min using an Optima MAX- XP Benchtop Ultracentrifuge (Beckman- Coulter) TLA- 100.3 rotor (k- Factor 14).

    Fluorescence:

    Article Title: Monitoring glioblastoma extracellular vesicle evolution using a nanodiagnostic platform to detect glioma stem cells driving recurrent disease.
    Article Snippet: .. Positive particles were gated based on a negative control of PBS or the respective fluorescent antibody to adjust for background fluorescence. sEV immunofluorescent staining for nanoFCM Approximately 1 × 109 total sEV particles were incubated with either anti- ATP1B2 (PA526279, Thermo Fisher Scientific), anti- EAAT2 (NOVNBP120136, Novus), anti- CD9 APC (17- 0098- 42, Thermo Fisher Scientific), anti- CD24 (ab134375, Abcam), anti- EGFR (ab231, Abcam), anti- CD44 Alexa Fluor 488 (103016, BioLegend), or antiCD133 Alexa Fluor 488 (FAB11331G, R&D Systems) in 50 μl of PBS, for 1 hour at 37°C. .. Labeled sEVs were diluted with 1 ml of PBS and ultracentrifuged at 110,000g for 30 min using an Optima MAX- XP Benchtop Ultracentrifuge (Beckman- Coulter) TLA- 100.3 rotor (k- Factor 14).

    Staining:

    Article Title: Monitoring glioblastoma extracellular vesicle evolution using a nanodiagnostic platform to detect glioma stem cells driving recurrent disease.
    Article Snippet: .. Positive particles were gated based on a negative control of PBS or the respective fluorescent antibody to adjust for background fluorescence. sEV immunofluorescent staining for nanoFCM Approximately 1 × 109 total sEV particles were incubated with either anti- ATP1B2 (PA526279, Thermo Fisher Scientific), anti- EAAT2 (NOVNBP120136, Novus), anti- CD9 APC (17- 0098- 42, Thermo Fisher Scientific), anti- CD24 (ab134375, Abcam), anti- EGFR (ab231, Abcam), anti- CD44 Alexa Fluor 488 (103016, BioLegend), or antiCD133 Alexa Fluor 488 (FAB11331G, R&D Systems) in 50 μl of PBS, for 1 hour at 37°C. .. Labeled sEVs were diluted with 1 ml of PBS and ultracentrifuged at 110,000g for 30 min using an Optima MAX- XP Benchtop Ultracentrifuge (Beckman- Coulter) TLA- 100.3 rotor (k- Factor 14).

    Incubation:

    Article Title: Monitoring glioblastoma extracellular vesicle evolution using a nanodiagnostic platform to detect glioma stem cells driving recurrent disease.
    Article Snippet: .. Positive particles were gated based on a negative control of PBS or the respective fluorescent antibody to adjust for background fluorescence. sEV immunofluorescent staining for nanoFCM Approximately 1 × 109 total sEV particles were incubated with either anti- ATP1B2 (PA526279, Thermo Fisher Scientific), anti- EAAT2 (NOVNBP120136, Novus), anti- CD9 APC (17- 0098- 42, Thermo Fisher Scientific), anti- CD24 (ab134375, Abcam), anti- EGFR (ab231, Abcam), anti- CD44 Alexa Fluor 488 (103016, BioLegend), or antiCD133 Alexa Fluor 488 (FAB11331G, R&D Systems) in 50 μl of PBS, for 1 hour at 37°C. .. Labeled sEVs were diluted with 1 ml of PBS and ultracentrifuged at 110,000g for 30 min using an Optima MAX- XP Benchtop Ultracentrifuge (Beckman- Coulter) TLA- 100.3 rotor (k- Factor 14).



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    94
    R&D Systems anticd133 alexa fluor 488
    Anticd133 Alexa Fluor 488, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anticd133+alexa+fluor+488/Human+CD133+Alexa+Fluor%C2%AE+488-conjugated+Antibody/pm41616062-259-72-77
    Average 94 stars, based on 1 article reviews
    anticd133 alexa fluor 488 - by Bioz Stars, 2026-09
    94/100 stars
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